Here are the sources and chemical tests for Indian gum, pectin, black catechu, ricinous oil, and gelatin:
1. Indian Gum (Gum Acacia)
Source:
- Derived from the sap of Acacia trees, primarily Acacia senegal and Acacia seyal.
Chemical Tests:
- Solubility Test: Dissolve a small amount of gum in water. It should form a viscous solution.
- Mucilage Test: Add a few drops of the solution to a glass of water. It should form a slimy, mucilaginous mass.
- Molisch’s Test: Add a few drops of Molisch’s reagent (alpha-naphthol solution in alcohol) and then a few drops of concentrated sulfuric acid along the side of the test tube. A purple or reddish-violet ring at the interface indicates the presence of carbohydrates.
2. Pectin
Source:
- Extracted from the cell walls of fruits, especially apples and citrus fruits.
Chemical Tests:
- Gel Formation Test: Mix pectin with sugar and acidic solution (like lemon juice) and heat. It should form a gel-like structure upon cooling.
- Alcohol Precipitation Test: Add ethanol to an aqueous solution of pectin. Pectin will precipitate out as a white gel.
- Color Reaction with Carbazole: Add a few drops of carbazole solution and concentrated sulfuric acid to the pectin solution. A red color indicates the presence of pectin.
3. Black Catechu (Cutch)
Source:
- Extracted from the heartwood of Acacia catechu.
Chemical Tests:
- Ferric Chloride Test: Add a few drops of ferric chloride solution to an aqueous solution of black catechu. A greenish-black or blue-black color indicates the presence of tannins.
- Goldbeater’s Skin Test: Soak a piece of goldbeater's skin in the catechu solution, wash with distilled water, and then immerse in a 1% solution of ferrous sulfate. A blue-black coloration indicates tannins.
- Vanillin-HCl Test: Add vanillin solution in concentrated hydrochloric acid to the catechu solution. A pink or red color indicates the presence of catechins.
4. Ricinous Oil (Castor Oil)
Source:
- Extracted from the seeds of the Ricinus communis plant.
Chemical Tests:
- Solubility Test: Ricinous oil is soluble in alcohol and chloroform but not in water.
- Baudouin Test: Mix the oil with an equal volume of hydrochloric acid and a few drops of 2% solution of phloroglucinol in alcohol. A pink color indicates the presence of castor oil.
- Hydroxyl Value Test: This test measures the hydroxyl content in the oil, which is high for ricinous oil due to the presence of ricinoleic acid.
5. Gelatin
Source:
- Obtained by hydrolysis of collagen, found in animal bones, skins, and connective tissues.
Chemical Tests:
- Biuret Test: Add a few drops of sodium hydroxide and a few drops of copper sulfate solution to the gelatin solution. A violet or pink color indicates the presence of peptide bonds.
- Xanthoproteic Test: Add concentrated nitric acid to the gelatin solution and heat. A yellow color forms, which turns orange upon adding ammonia, indicating the presence of aromatic amino acids.
- Gel Test: Dissolve gelatin in warm water and let it cool. It should form a gel upon cooling.

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